Date of Completion
2016
Document Type
Honors College Thesis
Department
Medical Laboratory and Radiation Sciences
Thesis Type
Honors College
First Advisor
Eyal Amiel
Second Advisor
Rory Waterman
Keywords
Dendritic cells, inflammasome, Dectin-1, immune, vaccine, metabolic regulation
Abstract
Dendritic cells (DCs) are critical antigen presenting cells that link the innate and adaptive immune systems. DCs are activated through a variety of receptors and respond with a diverse array of metabolic changes that are not yet well understood. IL-1β is a key inflammatory cytokine produced by DCs when they are activated through both toll-like receptors and C-type lectin receptors. IL-1β is activated by the inflammasome signaling complex but how the inflammasome is controlled by glycolysis is not yet well understood. We demonstrate that DC activation through TLR or C-type lectin receptors induces a shift to aerobic glycolytic metabolism. We show that while the transcription of IL-1β in DCs activated through both these receptors is not under glycolytic control, whether or not translation of IL-1β is under glycolytic control remains unclear. These findings provide new information on Dectin-1 mediated metabolic reprogramming in DCs. Understanding the link between metabolic changes and Dectin-1-mediated DC activation has broad implications for improved vaccine design and clinical intervention to fungal infection.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Recommended Citation
Ojemann, Alexandra, "Dectin-1 Mediated Regulation of Dendritic Cell Metabolism" (2016). UVM Honors College Senior Theses. 112.
https://scholarworks.uvm.edu/hcoltheses/112