Date of Completion

2016

Document Type

Honors College Thesis

Department

Medical Laboratory and Radiation Sciences

Type of Thesis

Honors College

First Advisor

Eyal Amiel

Second Advisor

Rory Waterman

Keywords

Dendritic cells, inflammasome, Dectin-1, immune, vaccine, metabolic regulation

Abstract

Dendritic cells (DCs) are critical antigen presenting cells that link the innate and adaptive immune systems. DCs are activated through a variety of receptors and respond with a diverse array of metabolic changes that are not yet well understood. IL-1β is a key inflammatory cytokine produced by DCs when they are activated through both toll-like receptors and C-type lectin receptors. IL-1β is activated by the inflammasome signaling complex but how the inflammasome is controlled by glycolysis is not yet well understood. We demonstrate that DC activation through TLR or C-type lectin receptors induces a shift to aerobic glycolytic metabolism. We show that while the transcription of IL-1β in DCs activated through both these receptors is not under glycolytic control, whether or not translation of IL-1β is under glycolytic control remains unclear. These findings provide new information on Dectin-1 mediated metabolic reprogramming in DCs. Understanding the link between metabolic changes and Dectin-1-mediated DC activation has broad implications for improved vaccine design and clinical intervention to fungal infection.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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