Date of Completion


Document Type

Honors College Thesis


Larner College of Medicine Department of Pathology and Laboratory Medicine

Thesis Type

Honors College, College of Arts and Science Honors

First Advisor

Yvonne Janssen-Heininger, Ph.D.

Second Advisor

Bryan Ballif, Ph.D.


IPF, glutaredoxin, differentiation, organoid


Idiopathic pulmonary fibrosis (IPF) is a deadly disease whose pathology involves improperly differentiated airway epithelia present in alveolar regions. Previous work from our group has shown decreased activity of the enzyme glutaredoxin (GLRX) in the lungs of IPF patients, and demonstrated reversal of lung fibrosis in mice via administration of exogenous GLRX protein. We modeled airway epithelial tissue using 3D cell culture of wild type and GLRX -/- airway epithelial basal cells, both in monoculture and in coculture with fibroblasts. We present data from qPCR and immunohistochemistry which suggest that GLRX is essential for the proper differentiation of airway epithelia in the presence of fibroblasts. These findings suggest a role for GLRX in the attenuation of fibrosis via the support of healthy epithelial differentiation.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.