Date of Completion
Honors College Thesis
College of Engineering and Mathematical Sciences
Type of Thesis
Dr. Rachael A. Oldinski
Dr. Darren L. Hitt
Dr. Frederic Sansoz
microfluidics, flow-focusing, silanization, silane, PDMS, poly(dimethylsiloxane), droplet generation, confocal, dripping, jetting, scaffold, arthritis
While the lifespan of humans has increased, the durability of cartilage has not, leading to increasing rates of arthritis in aging humans. As both natural and surgical methods for repairing osteochondral defects tend to fall short, UVM’s Engineered Biomaterials Research Laboratory (EBRL) is working towards a solution where biomimetic, polymeric, and porous engineered tissue scaffolds are seeded with drugs and human mesenchymal stem cells (hMSCs). The seeded scaffold is then implanted or injected into the patient’s osteochondral defect, where the hMSCs differentiate and grow a new cartilaginous extracellular matrix to heal the defect as the artificial scaffold breaks down.
Microspheres in three distinct size ranges are required to create pores and embed drugs and cells in the scaffold. In order to produce these microspheres, we turn to the field of microfluidics, which examines fluid interactions at micro-scale geometries and flow rates. A microfluidic flow-focusing device (MFFD) leverages the low Reynolds numbers and pronounced effects of surface tension in such flows to create highly monodisperse droplets of one fluid in a second.
This project investigates the design and fabrication of MFFDs for the production of homogeneous microspheres. A MFFD must be consistently reproducible, readily characterized, and easy to test and use. MFFDs show great potential to successfully play a role in the EBRL’s investigation of engineered tissue scaffolds.
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Hotaling, Samuel, "Design and Fabrication of Flow-Focusing Devices for Tissue Engineering Applications" (2015). UVM Honors College Senior Theses. 54.