Ergothioneine in an Enzyme: Using Protein Engineering to Create Unique Antioxidant Enzymes Containing 2-thiohistidine
Hassett, Elyse
Hassett, Elyse
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Abstract
Ergothioneine is a natural sulfur containing amino acid and a unique antioxidant compound. Ergothioneine is unable to be incorporated into a peptide because its nitrogen is trimethylated; however, 2-thiohistidine is an analogue of ergothioneine with similar antioxidant properties that can be inserted into a peptide/protein. This project utilizes protein engineering to replace a catalytic cysteine residue of Plasmodium falciparum thioredoxin reductase (PfTrxR)with 2-thiohistidine and investigates gains in function in three main mutants: PfTrxR-CGGGK2THG, PfTrxR-CUGGK2THG, and PFTrxR-CG2THKPG2THK. Our data supports the protective effects of 2-thiohistidine on the redox-active residue selenocysteine and the potential conferral of the ability to metabolize 1O2.
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Undergraduate
Date
2023-01-01