Cold and Drought Response Pathways of Brachypodium distachyon Through Analysis of Differentially Expressed Genes of GF14‐h and HDAC‐19 Mutants
Lantigua, Ashley
Lantigua, Ashley
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Abstract
Understanding the genetic basis of stress resilience is essential for breeding crops adapted to our changing climate. Based on their co‐expression during cold conditions, we created CRISPR mutants of HISTONE DEACETYLASE19 (HDAC19) and the 14‐3‐3 protein‐encoding gene GF14h in the temperate model grass Brachypodium distachyon. Transcriptome analyses from wild‐type and mutant shoots grown under drought conditions revealed HDAC19 and GF14h share a significant number of differentially expressed genes (DEGs). Furthermore, gene ontology enrichment analysis revealed an overrepresentation of stress response and metabolic process DEGs, supporting our hypothesis that HDAC19 and GF14h at least partially function in the same pathway
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Graduate
Date
2023-01-01