Effects of Thyroid Hormone Receptor Beta Selective Drug on Breast Cancer Cell Transcriptome
McFarland, Sydney
McFarland, Sydney
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Abstract
Breast cancer (BCa) is the most diagnosed cancer in women worldwide and the second leading cause of cancer death in the US. Estrogen receptor positive (ER+) BCa is marked by estrogen receptor alpha, a known tumor promoter that activates the expression of oncogenes. Thyroid Hormone Receptor Beta (TRb) is a nuclear receptor and transcription factor, and when activated can induce re-differentiation and tumor suppression in BCa. The Carr lab has shown that the activation of TRb with the synthetic agonist GC-1 can reduced tumor sphere formation, yet the effects of GC-1 on the transcriptome in ER+ BCa cells have not been previously shown. I conducted an RNA-seq experiment to investigate the differential expression of genes in response to GC-1 and thyroid hormone treatment. While the experiment didn’t yield conclusive results between treatments, I was able to learn the experimental design and data analysis process for RNAseq. This provides a framework for future RNAseq and other sequencing experiments.
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Undergraduate
Date
2024-01-01