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Project of Mixed Species Biofilms of Ralstonia and Chryseobacterium

Zhao, Zirui
; 
Kinahan, Matthew
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Abstract
The purpose of this project was to understand how Chryseobacterium gleum spp. and Ralstonia insidiosa spp. interact with each other to form a synergistic biofilm. A set of dual-species biofilm formation assays were performed. The dual-species biofilm formation assays consisted of Chryseobacterium gleum and Ralstonia insidiosa and Ralstonia pickettii, each bacterial strain was also cultured individually. The experiment was carried out by crossing a Chryseobacterium gleum strain with separate Ralstonia insidiosa strains and vice-versa from samples obtained from the international space station (ISS) potable water delivery system. A crystal violet staining protocol was used to visualize and later measure the biofilm formation. The implications of the research can be used to find an effective way to sanitize the potable water system on the ISS.
Description
Date
2021-01-01
Student Status
Undergraduate
Journal Title
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Volume Title
Type of presentation
Oral Paper Presentation
Collections
UVM Student Research Conference
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Citation
URI
https://hdl.handle.net/20.500.14849/8076
DOI
Advisor(s)
Matthew Wargo
Department
Program/Major
Biological Science
College/School
College of Arts and Sciences
Organization
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Biological Sciences
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