Optimization of an Embryonic Zebrafish Xenograft Model of Metastasis
Bialek, Lauren
Bialek, Lauren
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Abstract
The zebrafish xenograft model is valuable for studying cancer metastasis but lacks temperature standardization. Thus, our study aimed to establish an optimal temperature and migratory controls. Wild-type zebrafish embryos incubated at 34°C, 35°C and 28.5°C showed significantly lower mortality at 28.5°C after 6 days. While 34°C displayed significant mortality, embryo morphology was not impacted as seen at 35°C, consistent with previous findings. Fluorescently labeled human cancer cells are currently being injected into transgenic zebrafish embryos and imaged at 1- and 5-days post-injection to quantify extravasation. This will aid in exploring the impact of mutations like STK11 on lung adenocarcinoma metastasis.
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Undergraduate
Date
2024-01-01