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Identification of Abl-Induced Phosphorylation Sites on WBP2 and Abl- Dependent WBP2 Binding Partners
Girardet, Meg Boyer
Girardet, Meg Boyer
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Abstract
WW domain binding protein 2 (WBP2) is considered an emerging gene involved in the regulation of breast cancer primarily due to its known ability to act as an estrogen receptor transcriptional coactivator. WBP2 contains 11 YXXP (tyrosine-X-X-proline) amino acid motifs and these are essential to WBP2’s Abl-induced binding to the CrkL-SH2 domain. It is hypothesized that Abl induces WBP2 tyrosine phosphorylation in YXXP motifs driving its interaction with CrkL and additional proteins. Using cell culture, immunoprecipitation, immunoblotting, and mass spectrometry techniques, we have identified Abl-induced tyrosine phosphorylation sites on WBP2 and have identified binding partners to WBP2, including Abl- regulated binding partners.
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2025-01-01