Impact of Formulation on mRNA Lipid-nanoparticle Characteristics
Zuchowski, Adam
Zuchowski, Adam
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Abstract
Lipid nanoparticles are rapidly emerging as a new and powerful method of treatment for delivering drug therapies such as mRNA vaccines. Here, we have developed a rapid, scalable microfluidic strategy to encapsulate mRNA into lipid nanoparticles at laboratory scale. Whereas existing methods require large quantities of mRNA, this strategy enables production of uniform mRNA/lipid nanoparticles available for small-scale production in a research environment or in a limited resource setting. Using dynamic light scattering, encapsulation studies, and transmission electron microscopy, nanoparticles created using this novel microfluidic method is shown to produce uniform nanoparticles with high encapsulation efficiencies. Using these tools, feasibility of production of mRNA containing lipid nanoparticles is greatly increased in all settings, providing a solution for in vivo preclinical and resource limited clinical studies.
Description
Undergraduate
Date
2022-01-01