Negative regulation of hypoxia-inducible factor (HIF-1α) by the transcription factor RUNX1 in breast cancer cells
Herr, Lauren M
Herr, Lauren M
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Abstract
Loss of the transcription factor RUNX1 leads to breast cancer cell growth and expression of hypoxia-regulated genes. Our hypothesis is that RUNX1 is a negative regulator of hypoxia-inducible factor-1 (HIF-1α). MCF10A breast cancer cells containing degron-tagged RUNX1 were used to measure effects of RUNX1 degradation. Nuclear fractions were analyzed by Western blot and probed using antibodies for HIF-1α, RUNX1, and a histone control. Although RUNX1 degradation was successful, no significant change in HIF-1α stabilization in normoxia was observed at 4 or 24 hours. Because HIF-1α has a short half-life in normoxia, future experiments will test the effect at additional timepoints.
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Undergraduate
Date
2022-01-01