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Effects of isoleucine on the biosynthesis of branched-chain fatty acids by ruminal microorganisms

Matthews, Lily
; 
Kraft, Jana
; 
Vedovatto, Marcelo
; 
Greenwood, Sabrina
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Abstract
The objective of this study was to evaluate increasing doses of isoleucine, the metabolic precursor of the BCFAs anteiso-15:0 and anteiso-17:0, by ruminal microorganisms in vitro. Four single-flow continuous culture fermenters were utilized in a 4 X 4 Latin square design. Treatments consisted of 0.65 g isoleucine/day (baseline amount in the diet), 1.30 g/day, 1.95 g/day and 2.60 g/day. Bacterial and protozoal cell fractions were isolated from rumen fluid contents, and BCFA content of their lipid membranes was determined via gas-liquid chromatography. Our results provide direct evidence that rumen bacteria and protozoa use isoleucine as a precursor for anteiso-15:0.
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Graduate
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2022-01-01
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UVM Student Research Conference
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https://hdl.handle.net/20.500.14849/8366
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