Transcriptome Storage: TimeVault
Paup, Sienna
Paup, Sienna
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Abstract
Most molecular measurements of biological processes remain limited in their ability to preserve the dynamic that allows them to view information. A system that can preserve an unbiased record of the transcriptome within the same cellular lineage over time is needed. Ideally a system that can store past transcriptional states intracellularly in a format that can be recovered in daughter cells would solve many genome editing and RNA record issues and improve the longevity of a cell. This is where TimeVault comes in, a genetically encoded system that records and stores transcriptomes within living mammalian cells for future readout. It consists of engineered vault particles that capture mRNA through poly(A) binding proteins and demonstrates that the transcriptome stored by TimeVaults is stable in living cells for over 7 days, which is especially impressive considering current methods of preserving mRNA consist of freezing it to increase longevity of its half-life which is a maximum of twelve hours without TimeVault. The preservation mRNA is not the only benefit however, as it records the natural sequence with minimal cellular perturbation and preserving it before degradation of environmental exposure. It freezes the information, thus TimeVault.
Description
Date
1/1/2026
Student Status
Sophomore (Graduating in 2028)
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Poster
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Program/Major
Biology
College/School
College of Agriculture and Life Sciences
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Life Science