Entrapment of pyruvate dehydrogenase in alginate hydrogel sheets towards a biofilm-eliminating wound dressing
Sedighi, Omid
Sedighi, Omid
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Abstract
Biofilm infections challenge wound management due to their tolerence to antimicrobial treatments. Given pyruvate's role in biofilm formation, we developed a novel hydrogel wound dressing, encapsulating pyruvate dehydrogenase (PDH) within varying concentrations of alginate hydrogel to deplete pyruvate, aiming to disperse biofilms. The hydrogel demonstrated desirable mechanical properties and swelling capacity, essential for absorbing wound exudate and facilitating pyruvate-PDH interaction. Hydrogel’s structure was studied by scanning electron microscopy. Preliminary results indicate that the encapsulated PDH successfully depletes pyruvate, showing promise in controlling biofilm infections in wounds. Further research will focus on optimizing PDH concentration and conducting in-vitro studies.
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Graduate
Date
2024-01-01