Implementing VirScan: a Phage Display Library for Viral Antibody Detection and Characterization
Kjendal, Lily
Kjendal, Lily
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Abstract
Using VirScan, a PhIP-seq peptide library, serological studies looking at antibody repertoires against the entire human virome are possible. By implementing and modifying existing PhIP-seq methods to produce data that is replicable and reliable, such libraries like VirScan can be used to test a multitude of future questions including vaccine efficacy and epitope spread. Through this work, the effectiveness of the existing PhIP-seq method accompanying the VirScan peptide library was tested. The peptide library was amplified and quantified to ensure sufficient diversity was maintained, phage-antibody complexes were formed and precipitated, and captured phage DNA was sequenced. The resulting sequencing data was then analyzed, and peptide hits were calculated using relative z-scores. Through this method, a diverse peptide library was maintained, and peptides hits for Herpesvirus were identified, functioning as a positive control. However, the results were not consistent in sample replicates and positive control samples using monoclonal antibodies did not produce the expected results. This shows a problem of data noise in the existing method and presents the need for protocol adaptation before useful implementation can be achieved.
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Date
2024-01-01
Student Status
Undergraduate
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Oral Paper Presentation
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Molecular Genetics
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College of Agriculture and Life Sciences
Patrick Leahy Honors College
Patrick Leahy Honors College
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Life Sciences