Effects of inflammation on the gene expression of Parkinson's Disease linked LRRK2
Labuschagne, Bianca
Labuschagne, Bianca
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Abstract
Parkinson’s Disease (PD) is a neurodegenerative disease that can be triggered by inflammation. Expression of the LRRK2 gene, the most commonly mutated gene in PD, increases in response to inflammation; However, nothing is known about the specific DNA elements that are responsible. Molecular cloning, including PCR and HindIII/NheI digests, was used to clone LRRK2 promoter fragments, which would be used to identify specific DNA promotor regions required for inflammation-induced gene expression. Sequencing results revealed that the cloning was unsuccessful; These results are not consistent with previous studies in which the LRRK2 gene promotor was successfully cloned using HindIII/NheI digests.
Description
Undergraduate
Date
2023-01-01