Repeated variate stress increased voiding frequency and altered TrpV1 and TrpV4 transcript expression in lower urinary tract (LUT) pathways in female mice
Sidwell, Amanda McClintock, Celia
Sidwell, Amanda
McClintock, Celia
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Abstract
Interstitial cystitis/bladder pain syndrome (IC/BPS) affects quality of life and voiding function. Common symptoms are increased urgency, voiding frequency and pelvic pain. The etiology and underlying mechanisms of IC/BPS continue to be defined; however, psychological stress can exacerbate the symptoms of IC/BPS. Transientreceptor potential (TRP) channelsmay be potentialtargetsfor intervention due to their distribution in the LUT and role in pain. Here, we examine a model ofrepeated variate stress(RVS) and its effects on bladder function, anxiety-like behavior, and TRP transcript expression in urinary bladder and lumbosacral spinal cord and associated dorsal root ganglia (DRG). Using continuous infusion, open-outlet cystometry in conscious mice, RVS significantly (p ≤ 0.05) decreased infused volume and intermicturition interval. Quantitative PCR demonstrated significant (p ≤ 0.05) changes in TrpV1 and TrpV4 mRNA expression. Future directions will examine the contribution of TRP channels on bladder function, somatic sensation and anxiety-like behavior using the RVS model.
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Graduate
Date
2023-01-01