Impact of STK11 loss-of-function mutations on the metastatic potential of KRAS-mutated lung-adenocarcinoma
Royer, Cole
Royer, Cole
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Abstract
This study aimed to determine the impact of STK11 loss-of-function mutations on the metastatic potential in KRAS-mutated lung adenocarcinomas. The metastatic potential of STK11 loss-of-function lines were assessed utilizing migration as well as invasion assays. H2009 and ∆STK11, previously generated by CRISPR/Cas9 were utilized. ∆STK11 cells displayed a significant increase in migration compared to the parental control (n=6; p<0.0001). The invasive properties of ∆STK11 cells were assessed utilizing spheroids embedded in Matrigel matrix. The area of invasion in 24 hours was significantly larger in ∆STK11 cells compared to the parental control (n=19-21; p<0.0001).
Description
Undergraduate
Date
2024-01-01