Primary Faculty Mentor Name

Rachael Oldinski

Secondary Mentor NetID

pncharro

Status

Undergraduate

Student College

College of Engineering and Mathematical Sciences

Program/Major

Biomedical Engineering

Primary Research Category

Engineering & Physical Sciences

Secondary Research Category

Biological Sciences

Presentation Title

HUVEC Tubular Formation on Bio-inspired Vascularization Substrate

Time

9:00 AM

Location

Silver Maple Ballroom - Engineering & Physical Sciences

Abstract

While possessing many desired properties for a biomaterial capable of cell encapsulation, alginate lacks the ability to interact with mammalian cells; however, alginate can be chemically modified with RGD- peptides, which promote cell adhesion, proliferation and ingrowth. Heparin, a glycosaminoglycan naturally found in the body, plays a role in preventing the formation of a thrombosis due to its anticoagulant activity and is a component of the extracellular matrix of blood vessels that has been shown to promote the growth of endothelial cells in vitro. The aim of this study was to determine the feasibility heparin and arginyl-glycyl-aspartic acid (RGD) conjugated alginate hydrogels encapsulated with vascular endothelial growth factor (VEGF) as a wound dressing to promote vascularization. VEGF is an important component in angiogenesis that binds to heparin, and RGD promotes cell adhesion. Alginate hydrogels consisted of methacrylic anhydride (MA), RGD, and heparin, and were crosslinked with visible light. The material properties and burst pressure mechanics were evaluated to determine the structural integrity and adhesiveness of the hydrogels.

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HUVEC Tubular Formation on Bio-inspired Vascularization Substrate

While possessing many desired properties for a biomaterial capable of cell encapsulation, alginate lacks the ability to interact with mammalian cells; however, alginate can be chemically modified with RGD- peptides, which promote cell adhesion, proliferation and ingrowth. Heparin, a glycosaminoglycan naturally found in the body, plays a role in preventing the formation of a thrombosis due to its anticoagulant activity and is a component of the extracellular matrix of blood vessels that has been shown to promote the growth of endothelial cells in vitro. The aim of this study was to determine the feasibility heparin and arginyl-glycyl-aspartic acid (RGD) conjugated alginate hydrogels encapsulated with vascular endothelial growth factor (VEGF) as a wound dressing to promote vascularization. VEGF is an important component in angiogenesis that binds to heparin, and RGD promotes cell adhesion. Alginate hydrogels consisted of methacrylic anhydride (MA), RGD, and heparin, and were crosslinked with visible light. The material properties and burst pressure mechanics were evaluated to determine the structural integrity and adhesiveness of the hydrogels.