Presenter's Name(s)

Elaine Judith HasenohrFollow

Primary Faculty Mentor Name

Seth Frietze

Project Collaborators

Diana Gerrard (Graduate Student Mentor), Michael Mariani (Graduate student collaborating mentor)

Graduate Student Mentors

Diana Gerrard, Michael Mariani

Status

Undergraduate

Student College

College of Agriculture and Life Sciences

Program/Major

Microbiology and Molecular Genetics

Primary Research Category

Biological Sciences

Presentation Title

Genomic organization of the herpesviral genome within the host cell nucleus

Time

11:00 AM

Location

Silver Maple Ballroom - Biological Sciences

Abstract

Human herpesvirus-6 (HHV-6) is a ubiquitous human pathogen that is associated with seizures and encephalitis as well as various other diseases. HHV-6 has been recently shown to integrate its genome into the telomeres of latently infected cells. Upon integration, the virus genome is transcriptionally silenced. While epigenetic modifications including post-translational modification of histone proteins are known to play a critical role in herpesvirus latency, little is known regarding the role of chromatin structures in viral gene regulation and latency. I will test the hypothesis that the HHV-6 integrated genome forms specific genomic structures within the host cell nucleus to explore how chromatin interactions contribute to HHV-6 gene regulation and latency. While epigenetic mechanisms are critical to herpesvirus life cycles, little is known regarding their higher order chromatin structures and how these contribute to viral latency. Accordingly, I have employed circular chromosome conformation capture coupled with high-throughput sequencing (4C-seq analysis) to map the genome-wide maps of integrated HHV-6 genomes. Overall, this approach will facilitate the analysis of higher order chromatin structures formed by integrated HHV-6 genomes within latently infected cells.

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Genomic organization of the herpesviral genome within the host cell nucleus

Human herpesvirus-6 (HHV-6) is a ubiquitous human pathogen that is associated with seizures and encephalitis as well as various other diseases. HHV-6 has been recently shown to integrate its genome into the telomeres of latently infected cells. Upon integration, the virus genome is transcriptionally silenced. While epigenetic modifications including post-translational modification of histone proteins are known to play a critical role in herpesvirus latency, little is known regarding the role of chromatin structures in viral gene regulation and latency. I will test the hypothesis that the HHV-6 integrated genome forms specific genomic structures within the host cell nucleus to explore how chromatin interactions contribute to HHV-6 gene regulation and latency. While epigenetic mechanisms are critical to herpesvirus life cycles, little is known regarding their higher order chromatin structures and how these contribute to viral latency. Accordingly, I have employed circular chromosome conformation capture coupled with high-throughput sequencing (4C-seq analysis) to map the genome-wide maps of integrated HHV-6 genomes. Overall, this approach will facilitate the analysis of higher order chromatin structures formed by integrated HHV-6 genomes within latently infected cells.