Presentation Title

Dcbld2 is Essential in Zebrafish Retinal Development

Presenter's Name(s)

Jacqueline A. GuilleminFollow

Project Collaborators

Ryan Joy( PhD Graduate), Caitlin Hunt (Undergraduate) Alicia Ebert ( Mentor), Bryan Ballif ( Mentor)

Abstract

DCBLD2 is a type 1 transmembrane receptor that is expressed throughout the nervous system. Its structure resembles Neuropilins, which are co-receptors for the Semaphorin (Sema) family of repulsive guidance cues. Furthermore, DCBLD2 can bind to Sema4B and thus has features that suggest it could influence neuronal positioning and/or angiogenesis during development. Additionally, high DCBLD2 expression is linked to many forms of cancer, including glioma, neuroendocrine, lung and pancreatic cancer. While a role for DCBLD2 in angiogenesis has been described using morphant zebrafish, its function in the nervous system has yet to be elucidated. We determined that dcbld2 is expressed in the developing zebrafish visual system. We next disrupted dcbld2 expression using three approaches: (i) a slice-blocking morpholino (ii) CRISPR interference and (iii) a zebrafish mutant producing an aberrant Dcbld2 protein that truncates the protein in the ectodomain just prior to the transmembrane domain. In each approach retinal defects were observed. However, observed phenotypes were observed at different stages, to differing degrees, and in some cases unique to one disruption method. The data are discussed towards a model integrating the observations and leading to a better understanding of Dcbld2 function during eye development.

Primary Faculty Mentor Name

Alicia Ebert

Secondary Mentor NetID

Bballif

Secondary Mentor Name

Bryan Ballif

Graduate Student Mentors

Helaina Stergas, Caroline Dumas

Status

Graduate

Student College

Graduate College

Program/Major

Biology

Primary Research Category

Biological Sciences

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Dcbld2 is Essential in Zebrafish Retinal Development

DCBLD2 is a type 1 transmembrane receptor that is expressed throughout the nervous system. Its structure resembles Neuropilins, which are co-receptors for the Semaphorin (Sema) family of repulsive guidance cues. Furthermore, DCBLD2 can bind to Sema4B and thus has features that suggest it could influence neuronal positioning and/or angiogenesis during development. Additionally, high DCBLD2 expression is linked to many forms of cancer, including glioma, neuroendocrine, lung and pancreatic cancer. While a role for DCBLD2 in angiogenesis has been described using morphant zebrafish, its function in the nervous system has yet to be elucidated. We determined that dcbld2 is expressed in the developing zebrafish visual system. We next disrupted dcbld2 expression using three approaches: (i) a slice-blocking morpholino (ii) CRISPR interference and (iii) a zebrafish mutant producing an aberrant Dcbld2 protein that truncates the protein in the ectodomain just prior to the transmembrane domain. In each approach retinal defects were observed. However, observed phenotypes were observed at different stages, to differing degrees, and in some cases unique to one disruption method. The data are discussed towards a model integrating the observations and leading to a better understanding of Dcbld2 function during eye development.