Investigation of Potential Differential Mitochondrial Membrane Remodeling between Temperate and Tropical Drosophila melanogaster Populations

Presenter's Name(s)

Neel V. Patel, UVMFollow

Abstract

I investigated mitochondrial membrane fluidity between temperate (Vermont, USA) and tropical (St. Kitts) populations of D. melanogaster by subjecting them to development at lab acclimation of cold and warm temperatures. The cold temperature group were reared at 18°C while the warm temperature group were reared at 28°C. Their mitochondrial membrane fluidity was measured as adults after eclosing using the fluorescence probe 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH) and fluorescence polarization (FP). I found that while St. Kitts flies had higher mitochondrial membrane rigidity in both treatments, the Vermont flies showed great phenotypic plasticity in being able to be better remodel their mitochondrial membranes.

Primary Faculty Mentor Name

Dr. Brent Lockwood

Graduate Student Mentors

thomas.oleary

Faculty/Staff Collaborators

Dr. Brent Lockwood (Thesis advisor), Dr. Emily Mikucki (Post-doc Mentor), Dr. Sumaetee Tangwancharoen (Post-doc mentor), Thomas O'Leary (Graduate Student Mentor)

Status

Undergraduate

Student College

College of Arts and Sciences

Program/Major

Biological Science

Primary Research Category

Biological Sciences

Abstract only.

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Investigation of Potential Differential Mitochondrial Membrane Remodeling between Temperate and Tropical Drosophila melanogaster Populations

I investigated mitochondrial membrane fluidity between temperate (Vermont, USA) and tropical (St. Kitts) populations of D. melanogaster by subjecting them to development at lab acclimation of cold and warm temperatures. The cold temperature group were reared at 18°C while the warm temperature group were reared at 28°C. Their mitochondrial membrane fluidity was measured as adults after eclosing using the fluorescence probe 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH) and fluorescence polarization (FP). I found that while St. Kitts flies had higher mitochondrial membrane rigidity in both treatments, the Vermont flies showed great phenotypic plasticity in being able to be better remodel their mitochondrial membranes.