Genome-wide association study in accessions of the mini-core collection of mungbean (Vigna radiata) from the World Vegetable Gene Bank (Taiwan)

Authors

Alena Sokolkova, Peter the Great St. Petersburg Polytechnic University
Marina Burlyaeva, N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources
Tatjana Valiannikova, Kuban Branch of Federal Research Centre All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR)
Margarita Vishnyakova, N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources
Roland Schafleitner, AVRDC - The World Vegetable Center
Cheng Ruei Lee, National Taiwan University
Chau Ti Ting, National Taiwan University
Ramakrishnan Madhavan Nair, World Vegetable Center
Sergey Nuzhdin, Peter the Great St. Petersburg Polytechnic University
Maria Samsonova, Peter the Great St. Petersburg Polytechnic University
Eric von Wettberg, Peter the Great St. Petersburg Polytechnic University

Document Type

Article

Publication Date

10-1-2020

Abstract

Background: Mungbean (Vigna radiata (L.) R. Wilczek, or green gram) is important tropical and sub-tropical legume and a rich source of dietary protein and micronutrients. In this study we employ GWAS to examine the genetic basis of variation in several important traits in mungbean, using the mini-core collection established by the World Vegetable Center, which includes 296 accessions that represent the major market classes. This collection has been grown in a common field plot in southern European part of Russia in 2018. Results: We used 5041 SNPs in 293 accessions that passed strict filtering for genetic diversity, linkage disequilibrium, population structure and GWAS analysis. Polymorphisms were distributed among all chromosomes, but with variable density. Linkage disequilibrium decayed in approximately 105 kb. Four distinct subgroups were identified within 293 accessions with 70% of accessions attributed to one of the four populations. By performing GWAS on the mini-core collection we have found several loci significantly associated with two important agronomical traits. Four SNPs associated with possibility of maturation in Kuban territory of Southern Russia in 2018 were identified within a region of strong linkage which contains genes encoding zinc finger A20 and an AN1 domain stress-associated protein. Conclusions: The core collection of mungbean established by the World Vegetable Center is a valuable resource for mungbean breeding. The collection has been grown in southern European part of Russia in 2018 under incidental stresses caused by abnormally hot weather and different photoperiod. We have found several loci significantly associated with color of hypocotyl and possibility of maturation under these stressful conditions. SNPs associated with possibility of maturation localize to a region on chromosome 2 with strong linkage, in which genes encoding zinc finger A20 and AN1 domain stress associated protein (SAP) are located. Phenotyping of WorldVeg collection for maturation traits in temperate climatic locations is important as phenology remains a critical breeding target for mungbean. As demand rises for mungbean, production in temperate regions with shorter growing seasons becomes crucial to keep up with needs. Uncovering SNPs for phenology traits will speed breeding efforts.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Rights Information

© 2020, The Author(s).

Recommended Citation

Sokolkova A, Burlyaeva M, Valiannikova T, Vishnyakova M, Schafleitner R, Lee CR, Ting CT, Nair RM, Nuzhdin S, Samsonova M, von Wettberg E. Genome-wide association study in accessions of the mini-core collection of mungbean (Vigna radiata) from the World Vegetable Gene Bank (Taiwan). BMC plant biology. 2020 Oct;20(1):1-9.

DOI

10.1186/s12870-020-02579-x