Date of Completion
2022
Document Type
Honors College Thesis
Department
Department of Animal and Veterinary Sciences
Thesis Type
Honors College
First Advisor
John Barlow
Keywords
Cinnamon bark oil, non-aureus staphylococci, bovine mastitis, antimicrobial susceptibility testing, essential oils
Abstract
Aims:
The aims of this study were to develop a method for antimicrobial susceptibility testing (AST) with cinnamon bark oil (CBO) by broth microdilution, to determine the minimum inhibitory concentration (MIC) of CBO for several non-aureus staphylococci isolates, and to determine whether there is a correlation between penicillin MIC and CBO MIC for these isolates.
Methods and Results:
AST for both CBO and penicillin was performed on four isolates of S. chromogenes, three isolates of S. equorum, and three isolates of S. xylosus.
Conclusions:
All isolates had a CBO MIC within the tested range of CBO concentrations. No correlation was found between penicillin MIC and CBO MIC for all isolates.
Significance and Impacts:
These findings demonstrate that CBO has antimicrobial activity against several species of non-aureusstaphylococci, including two bovine-mastitis-causing pathogens, supporting the possibility that it could be used in the dairy industry in the prevention and treatment of mastitis. The lack of correlation between CBO MIC and penicillin MIC suggests that CBO may be effective even against penicillin-resistant strains. Furthermore, a method was developed for performing AST with CBO by broth microdilution, which could be used in future work.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Recommended Citation
Dollar, Leah C., "The Antimicrobial Effects of Cinnamon Bark Essential Oil on Three Species of Non-aureus Staphylococci Associated with the Bovine Udder and Teat Skin" (2022). UVM Honors College Senior Theses. 460.
https://scholarworks.uvm.edu/hcoltheses/460