Designing a DNA Extraction Protocol to Yield High Quality DNA from S. purpuratus
Conference Year
January 2020
Abstract
The study focused on developing and tweaking a protocol for extracting high quality DNA from the sea urchin, Strongylocentrotus purpuratus, specifically from frozen tissues. The yields and quality of frozen individuals were compared to that of live individuals. Tissue from the frozen individuals were taken from the mouth, stomach, Aristotle's Lantern, tube feet, eggs, and sperm. Tissue from fresh individuals were taken from the tube feet and base of the spines. It was found that fresh tissue had much higher yields and quality than that of frozen tissue.
Primary Faculty Mentor Name
Melissa Pespeni
Faculty/Staff Collaborators
Csenge Petak (Graduate Student Mentor)
Status
Undergraduate
Student College
College of Agriculture and Life Sciences
Program/Major
Environmental Sciences
Second Program/Major
Microbiology and Molecular Genetics
Primary Research Category
Biological Sciences
Designing a DNA Extraction Protocol to Yield High Quality DNA from S. purpuratus
The study focused on developing and tweaking a protocol for extracting high quality DNA from the sea urchin, Strongylocentrotus purpuratus, specifically from frozen tissues. The yields and quality of frozen individuals were compared to that of live individuals. Tissue from the frozen individuals were taken from the mouth, stomach, Aristotle's Lantern, tube feet, eggs, and sperm. Tissue from fresh individuals were taken from the tube feet and base of the spines. It was found that fresh tissue had much higher yields and quality than that of frozen tissue.