Presentation Title

Designing a DNA Extraction Protocol to Yield High Quality DNA from S. purpuratus

Presenter's Name(s)

Mackenzie E. KernerFollow

Project Collaborators

Csenge Petak (Graduate Student Mentor)

Abstract

The study focused on developing and tweaking a protocol for extracting high quality DNA from the sea urchin, Strongylocentrotus purpuratus, specifically from frozen tissues. The yields and quality of frozen individuals were compared to that of live individuals. Tissue from the frozen individuals were taken from the mouth, stomach, Aristotle's Lantern, tube feet, eggs, and sperm. Tissue from fresh individuals were taken from the tube feet and base of the spines. It was found that fresh tissue had much higher yields and quality than that of frozen tissue.

Primary Faculty Mentor Name

Melissa Pespeni

Status

Undergraduate

Student College

College of Agriculture and Life Sciences

Program/Major

Environmental Sciences

Primary Research Category

Biological Sciences

Second Program/Major

Microbiology and Molecular Genetics

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Designing a DNA Extraction Protocol to Yield High Quality DNA from S. purpuratus

The study focused on developing and tweaking a protocol for extracting high quality DNA from the sea urchin, Strongylocentrotus purpuratus, specifically from frozen tissues. The yields and quality of frozen individuals were compared to that of live individuals. Tissue from the frozen individuals were taken from the mouth, stomach, Aristotle's Lantern, tube feet, eggs, and sperm. Tissue from fresh individuals were taken from the tube feet and base of the spines. It was found that fresh tissue had much higher yields and quality than that of frozen tissue.