Presentation Title
Project of Mixed Species Biofilms of Ralstonia and Chryseobacterium
Abstract
The purpose of this project was to understand how Chryseobacterium gleum spp. and Ralstonia insidiosa spp. interact with each other to form a synergistic biofilm. A set of dual-species biofilm formation assays were performed. The dual-species biofilm formation assays consisted of Chryseobacterium gleum and Ralstonia insidiosa and Ralstonia pickettii, each bacterial strain was also cultured individually. The experiment was carried out by crossing a Chryseobacterium gleum strain with separate Ralstonia insidiosa strains and vice-versa from samples obtained from the international space station (ISS) potable water delivery system. A crystal violet staining protocol was used to visualize and later measure the biofilm formation. The implications of the research can be used to find an effective way to sanitize the potable water system on the ISS.
Primary Faculty Mentor Name
Matthew Wargo
Faculty/Staff Collaborators
Kristin Schutz (Lab Manager)
Status
Undergraduate
Student College
College of Arts and Sciences
Program/Major
Biological Science
Primary Research Category
Biological Sciences
Project of Mixed Species Biofilms of Ralstonia and Chryseobacterium
The purpose of this project was to understand how Chryseobacterium gleum spp. and Ralstonia insidiosa spp. interact with each other to form a synergistic biofilm. A set of dual-species biofilm formation assays were performed. The dual-species biofilm formation assays consisted of Chryseobacterium gleum and Ralstonia insidiosa and Ralstonia pickettii, each bacterial strain was also cultured individually. The experiment was carried out by crossing a Chryseobacterium gleum strain with separate Ralstonia insidiosa strains and vice-versa from samples obtained from the international space station (ISS) potable water delivery system. A crystal violet staining protocol was used to visualize and later measure the biofilm formation. The implications of the research can be used to find an effective way to sanitize the potable water system on the ISS.