Presentation Title

Project of Mixed Species Biofilms of Ralstonia and Chryseobacterium

Project Collaborators

Kristin Schutz (Lab Manager)

Abstract

The purpose of this project was to understand how Chryseobacterium gleum spp. and Ralstonia insidiosa spp. interact with each other to form a synergistic biofilm. A set of dual-species biofilm formation assays were performed. The dual-species biofilm formation assays consisted of Chryseobacterium gleum and Ralstonia insidiosa and Ralstonia pickettii, each bacterial strain was also cultured individually. The experiment was carried out by crossing a Chryseobacterium gleum strain with separate Ralstonia insidiosa strains and vice-versa from samples obtained from the international space station (ISS) potable water delivery system. A crystal violet staining protocol was used to visualize and later measure the biofilm formation. The implications of the research can be used to find an effective way to sanitize the potable water system on the ISS.

Primary Faculty Mentor Name

Matthew Wargo

Status

Undergraduate

Student College

College of Arts and Sciences

Program/Major

Biological Science

Primary Research Category

Biological Sciences

This document is currently not available here.

Share

COinS
 

Project of Mixed Species Biofilms of Ralstonia and Chryseobacterium

The purpose of this project was to understand how Chryseobacterium gleum spp. and Ralstonia insidiosa spp. interact with each other to form a synergistic biofilm. A set of dual-species biofilm formation assays were performed. The dual-species biofilm formation assays consisted of Chryseobacterium gleum and Ralstonia insidiosa and Ralstonia pickettii, each bacterial strain was also cultured individually. The experiment was carried out by crossing a Chryseobacterium gleum strain with separate Ralstonia insidiosa strains and vice-versa from samples obtained from the international space station (ISS) potable water delivery system. A crystal violet staining protocol was used to visualize and later measure the biofilm formation. The implications of the research can be used to find an effective way to sanitize the potable water system on the ISS.