The effects of dietary peuNDF-240 and rumen fermentable starch on the milk proteome of dairy cows
Conference Year
January 2022
Abstract
Objectives were to investigate possible milk protein biomarkers and identify milk proteins affected by dietary physically effective undegraded neutral detergent fiber (peuNDF-240) and rumen fermentable starch (RFS) and determine if milking time relative to feeding affected these proteins. Milk samples were collected from sixteen Holsteins cows milked thrice daily (4:30, 12:30, and 20:30 h)fed either a high peuNDF240 high RFS diet or low peuNDF240 low RFS diet for 28 d. Milk proteins were analyzed using LC-MS/MS. Results were analyzed using PROC MIXED in SAS. Thirteen proteins were affected by time, treatment, and their interaction, including xanthine dehydrogenase/oxidase, and zinc-alpha-2-glycoprotein.
Primary Faculty Mentor Name
Dr. Sabrina Greenwood
Graduate Student Mentors
Bharath Kumar Mulakala, Miriam Snider
Status
Undergraduate
Student College
College of Agriculture and Life Sciences
Program/Major
Biological Science
Primary Research Category
Biological Sciences
The effects of dietary peuNDF-240 and rumen fermentable starch on the milk proteome of dairy cows
Objectives were to investigate possible milk protein biomarkers and identify milk proteins affected by dietary physically effective undegraded neutral detergent fiber (peuNDF-240) and rumen fermentable starch (RFS) and determine if milking time relative to feeding affected these proteins. Milk samples were collected from sixteen Holsteins cows milked thrice daily (4:30, 12:30, and 20:30 h)fed either a high peuNDF240 high RFS diet or low peuNDF240 low RFS diet for 28 d. Milk proteins were analyzed using LC-MS/MS. Results were analyzed using PROC MIXED in SAS. Thirteen proteins were affected by time, treatment, and their interaction, including xanthine dehydrogenase/oxidase, and zinc-alpha-2-glycoprotein.