Characterization of lncRNA U90926 and its protein coding potential
Conference Year
January 2022
Abstract
I am investigating the coding potential of novel mouse long non-coding RNA (lncRNA) U90926 (U9) based on off recent research that suggests lncRNAs may encode small but function proteins. U9’s open reading frame (ORF) contains a signal sequence that directs translation to the lumen of the ER. Through overexpression of tagged U9 ORF and immunocytochemistry, I determined that the U9 peptide is localized to the Golgi. Additionally, I confirmed that it follows this secretory pathway and is secreted via western blot. Current investigation is aimed at creating a monoclonal antibody to endogenously detect U9 and determining any post translational modifications.
Primary Faculty Mentor Name
Dimitry Krementsov
Graduate Student Mentors
Bristy Sabikunnahar
Status
Undergraduate
Student College
College of Agriculture and Life Sciences
Program/Major
Molecular Genetics
Primary Research Category
Biological Sciences
Characterization of lncRNA U90926 and its protein coding potential
I am investigating the coding potential of novel mouse long non-coding RNA (lncRNA) U90926 (U9) based on off recent research that suggests lncRNAs may encode small but function proteins. U9’s open reading frame (ORF) contains a signal sequence that directs translation to the lumen of the ER. Through overexpression of tagged U9 ORF and immunocytochemistry, I determined that the U9 peptide is localized to the Golgi. Additionally, I confirmed that it follows this secretory pathway and is secreted via western blot. Current investigation is aimed at creating a monoclonal antibody to endogenously detect U9 and determining any post translational modifications.