Characterization of lncRNA U90926 and its protein coding potential

Abstract

I am investigating the coding potential of novel mouse long non-coding RNA (lncRNA) U90926 (U9) based on off recent research that suggests lncRNAs may encode small but function proteins. U9’s open reading frame (ORF) contains a signal sequence that directs translation to the lumen of the ER. Through overexpression of tagged U9 ORF and immunocytochemistry, I determined that the U9 peptide is localized to the Golgi. Additionally, I confirmed that it follows this secretory pathway and is secreted via western blot. Current investigation is aimed at creating a monoclonal antibody to endogenously detect U9 and determining any post translational modifications.

Primary Faculty Mentor Name

Dimitry Krementsov

Graduate Student Mentors

Bristy Sabikunnahar

Status

Undergraduate

Student College

College of Agriculture and Life Sciences

Program/Major

Molecular Genetics

Primary Research Category

Biological Sciences

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Characterization of lncRNA U90926 and its protein coding potential

I am investigating the coding potential of novel mouse long non-coding RNA (lncRNA) U90926 (U9) based on off recent research that suggests lncRNAs may encode small but function proteins. U9’s open reading frame (ORF) contains a signal sequence that directs translation to the lumen of the ER. Through overexpression of tagged U9 ORF and immunocytochemistry, I determined that the U9 peptide is localized to the Golgi. Additionally, I confirmed that it follows this secretory pathway and is secreted via western blot. Current investigation is aimed at creating a monoclonal antibody to endogenously detect U9 and determining any post translational modifications.