Effects of isoleucine on the biosynthesis of branched-chain fatty acids by ruminal microorganisms
Conference Year
January 2022
Abstract
The objective of this study was to evaluate increasing doses of isoleucine, the metabolic precursor of the BCFAs anteiso-15:0 and anteiso-17:0, by ruminal microorganisms in vitro. Four single-flow continuous culture fermenters were utilized in a 4 X 4 Latin square design. Treatments consisted of 0.65 g isoleucine/day (baseline amount in the diet), 1.30 g/day, 1.95 g/day and 2.60 g/day. Bacterial and protozoal cell fractions were isolated from rumen fluid contents, and BCFA content of their lipid membranes was determined via gas-liquid chromatography. Our results provide direct evidence that rumen bacteria and protozoa use isoleucine as a precursor for anteiso-15:0.
Primary Faculty Mentor Name
Dr. Jana Kraft
Faculty/Staff Collaborators
Dr. Sabrina Greenwood
Status
Graduate
Student College
College of Agriculture and Life Sciences
Program/Major
Animal, Nutrition and Food Sciences
Primary Research Category
Biological Sciences
Effects of isoleucine on the biosynthesis of branched-chain fatty acids by ruminal microorganisms
The objective of this study was to evaluate increasing doses of isoleucine, the metabolic precursor of the BCFAs anteiso-15:0 and anteiso-17:0, by ruminal microorganisms in vitro. Four single-flow continuous culture fermenters were utilized in a 4 X 4 Latin square design. Treatments consisted of 0.65 g isoleucine/day (baseline amount in the diet), 1.30 g/day, 1.95 g/day and 2.60 g/day. Bacterial and protozoal cell fractions were isolated from rumen fluid contents, and BCFA content of their lipid membranes was determined via gas-liquid chromatography. Our results provide direct evidence that rumen bacteria and protozoa use isoleucine as a precursor for anteiso-15:0.