Repeated variate stress increased voiding frequency and altered TrpV1 and TrpV4 transcript expression in lower urinary tract (LUT) pathways in female mice
Conference Year
2023
Abstract
Interstitial cystitis/bladder pain syndrome (IC/BPS) affects quality of life and voiding function. Common symptoms are increased urgency, voiding frequency and pelvic pain. The etiology and underlying mechanisms of IC/BPS continue to be defined; however, psychological stress can exacerbate the symptoms of IC/BPS. Transientreceptor potential (TRP) channelsmay be potentialtargetsfor intervention due to their distribution in the LUT and role in pain. Here, we examine a model ofrepeated variate stress(RVS) and its effects on bladder function, anxiety-like behavior, and TRP transcript expression in urinary bladder and lumbosacral spinal cord and associated dorsal root ganglia (DRG). Using continuous infusion, open-outlet cystometry in conscious mice, RVS significantly (p ≤ 0.05) decreased infused volume and intermicturition interval. Quantitative PCR demonstrated significant (p ≤ 0.05) changes in TrpV1 and TrpV4 mRNA expression. Future directions will examine the contribution of TRP channels on bladder function, somatic sensation and anxiety-like behavior using the RVS model.
Primary Faculty Mentor Name
Margaret Vizzard
Status
Graduate
Student College
Larner College of Medicine
Second Student College
College of Arts and Sciences
Program/Major
Neuroscience
Primary Research Category
Life Sciences
Repeated variate stress increased voiding frequency and altered TrpV1 and TrpV4 transcript expression in lower urinary tract (LUT) pathways in female mice
Interstitial cystitis/bladder pain syndrome (IC/BPS) affects quality of life and voiding function. Common symptoms are increased urgency, voiding frequency and pelvic pain. The etiology and underlying mechanisms of IC/BPS continue to be defined; however, psychological stress can exacerbate the symptoms of IC/BPS. Transientreceptor potential (TRP) channelsmay be potentialtargetsfor intervention due to their distribution in the LUT and role in pain. Here, we examine a model ofrepeated variate stress(RVS) and its effects on bladder function, anxiety-like behavior, and TRP transcript expression in urinary bladder and lumbosacral spinal cord and associated dorsal root ganglia (DRG). Using continuous infusion, open-outlet cystometry in conscious mice, RVS significantly (p ≤ 0.05) decreased infused volume and intermicturition interval. Quantitative PCR demonstrated significant (p ≤ 0.05) changes in TrpV1 and TrpV4 mRNA expression. Future directions will examine the contribution of TRP channels on bladder function, somatic sensation and anxiety-like behavior using the RVS model.