The Effects of Mutations in a Highly Conserved Site on the Alpha-4 Helix of KIF18A
Conference Year
2023
Abstract
KIF18A is a multitalented mitotic kinesin that promotes chromosome alignment and maintains spindle shape. However, how KIF18A is regulated to perform its roles within the cell is not well understood. To investigate potential mechanisms of regulation, the functional effects of modifications at a conserved serine residue (S284) in the motor domain of KIF18A were examined. We found that mutations at S284 cause altered localization of KIF18A, chromosome unalignment, and spindle malformation. This work highlights the potential for modifications at this conserved motor domain residue to regulate kinesin function.
Primary Faculty Mentor Name
Jason Stumpff
Graduate Student Mentors
Katie Queen
Status
Undergraduate
Student College
College of Arts and Sciences
Second Student College
Patrick Leahy Honors College
Program/Major
Biology
Primary Research Category
Clinical
The Effects of Mutations in a Highly Conserved Site on the Alpha-4 Helix of KIF18A
KIF18A is a multitalented mitotic kinesin that promotes chromosome alignment and maintains spindle shape. However, how KIF18A is regulated to perform its roles within the cell is not well understood. To investigate potential mechanisms of regulation, the functional effects of modifications at a conserved serine residue (S284) in the motor domain of KIF18A were examined. We found that mutations at S284 cause altered localization of KIF18A, chromosome unalignment, and spindle malformation. This work highlights the potential for modifications at this conserved motor domain residue to regulate kinesin function.
