Immunogold Bioconjugate Nanoparticle Synthesis for Bacterial Targeting
Conference Year
2023
Abstract
Bacterial keratitis is a corneal infection that requiresstrict and time-consuming treatment, often involving repetitive antibiotic eye drop use. As an approach to developing a more efficient treatment, immunogold bioconjugate nanoparticles were synthesized for targeted labeling of Pseudomonas aeruginosa, a common pathogen resulting in keratitis. The nanoparticles were characterized utilizing size, surface charge, and UV/Visible spectroscopy absorbance. The antibody conjugated nanoparticles with poly(ethylene glycol) (PEG) coating showed an increase in diameter and a slight peak absorbance wavelength shift. Future work will involve further characterization of the particles and exposure to P. aeruginosa to observe the efficacy of the targeted labeling.
Primary Faculty Mentor Name
Amber Doiron
Status
Undergraduate
Student College
College of Engineering and Mathematical Sciences
Program/Major
Biomedical Engineering
Primary Research Category
Engineering and Math Science
Immunogold Bioconjugate Nanoparticle Synthesis for Bacterial Targeting
Bacterial keratitis is a corneal infection that requiresstrict and time-consuming treatment, often involving repetitive antibiotic eye drop use. As an approach to developing a more efficient treatment, immunogold bioconjugate nanoparticles were synthesized for targeted labeling of Pseudomonas aeruginosa, a common pathogen resulting in keratitis. The nanoparticles were characterized utilizing size, surface charge, and UV/Visible spectroscopy absorbance. The antibody conjugated nanoparticles with poly(ethylene glycol) (PEG) coating showed an increase in diameter and a slight peak absorbance wavelength shift. Future work will involve further characterization of the particles and exposure to P. aeruginosa to observe the efficacy of the targeted labeling.