Effects of inflammation on the gene expression of Parkinson's Disease linked LRRK2
Conference Year
January 2023
Abstract
Parkinson’s Disease (PD) is a neurodegenerative disease that can be triggered by inflammation. Expression of the LRRK2 gene, the most commonly mutated gene in PD, increases in response to inflammation; However, nothing is known about the specific DNA elements that are responsible. Molecular cloning, including PCR and HindIII/NheI digests, was used to clone LRRK2 promoter fragments, which would be used to identify specific DNA promotor regions required for inflammation-induced gene expression. Sequencing results revealed that the cloning was unsuccessful; These results are not consistent with previous studies in which the LRRK2 gene promotor was successfully cloned using HindIII/NheI digests.
Primary Faculty Mentor Name
John Salogiannis
Graduate Student Mentors
Ally Morrissey
Status
Undergraduate
Student College
College of Agriculture and Life Sciences
Program/Major
Molecular Genetics
Primary Research Category
Life Sciences
Effects of inflammation on the gene expression of Parkinson's Disease linked LRRK2
Parkinson’s Disease (PD) is a neurodegenerative disease that can be triggered by inflammation. Expression of the LRRK2 gene, the most commonly mutated gene in PD, increases in response to inflammation; However, nothing is known about the specific DNA elements that are responsible. Molecular cloning, including PCR and HindIII/NheI digests, was used to clone LRRK2 promoter fragments, which would be used to identify specific DNA promotor regions required for inflammation-induced gene expression. Sequencing results revealed that the cloning was unsuccessful; These results are not consistent with previous studies in which the LRRK2 gene promotor was successfully cloned using HindIII/NheI digests.