Cold and Drought Response Pathways of Brachypodium distachyon Through Analysis of Differentially Expressed Genes of GF14‐h and HDAC‐19 Mutants
Conference Year
2023
Abstract
Understanding the genetic basis of stress resilience is essential for breeding crops adapted to our changing climate. Based on their co‐expression during cold conditions, we created CRISPR mutants of HISTONE DEACETYLASE19 (HDAC19) and the 14‐3‐3 protein‐encoding gene GF14h in the temperate model grass Brachypodium distachyon. Transcriptome analyses from wild‐type and mutant shoots grown under drought conditions revealed HDAC19 and GF14h share a significant number of differentially expressed genes (DEGs). Furthermore, gene ontology enrichment analysis revealed an overrepresentation of stress response and metabolic process DEGs, supporting our hypothesis that HDAC19 and GF14h at least partially function in the same pathway
Primary Faculty Mentor Name
Jill Preston
Status
Graduate
Student College
College of Agriculture and Life Sciences
Second Student College
Graduate College
Program/Major
Plant Biology
Primary Research Category
Life Sciences
Cold and Drought Response Pathways of Brachypodium distachyon Through Analysis of Differentially Expressed Genes of GF14‐h and HDAC‐19 Mutants
Understanding the genetic basis of stress resilience is essential for breeding crops adapted to our changing climate. Based on their co‐expression during cold conditions, we created CRISPR mutants of HISTONE DEACETYLASE19 (HDAC19) and the 14‐3‐3 protein‐encoding gene GF14h in the temperate model grass Brachypodium distachyon. Transcriptome analyses from wild‐type and mutant shoots grown under drought conditions revealed HDAC19 and GF14h share a significant number of differentially expressed genes (DEGs). Furthermore, gene ontology enrichment analysis revealed an overrepresentation of stress response and metabolic process DEGs, supporting our hypothesis that HDAC19 and GF14h at least partially function in the same pathway