Whole Genome Sequencing Identifies Exotoxin and Antimicrobial Resistance Profiles of Staphylococcus aureus from Maine Dairy Farms

Presenter's Name(s)

Elisabeth Roadcap

Conference Year

2024

Abstract

Staphylococcus aureus is a bacterial pathogen which causes mastitis in dairy livestock. This study described retrospective trends of S. aureus detection in milk samples submitted between July 2017 and June 2022 to the University of Maine Cooperative Extension Veterinary Diagnostic Laboratory and assessed genomes of 29 S. aureus isolates. S. aureus was detected in 7.8% of milk samples from the observed period. The 29 genomes belonged to 8 strain types and 5 clonal complexes and contained antimicrobial resistance and exotoxin genes which mostly clustered with clonal complexes and species of origin. Future research should assess S. aureus prevalence in Maine.

Primary Faculty Mentor Name

John Barlow

Status

Undergraduate

Student College

College of Agriculture and Life Sciences

Second Student College

Patrick Leahy Honors College

Program/Major

Molecular Genetics

Primary Research Category

Life Sciences

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Whole Genome Sequencing Identifies Exotoxin and Antimicrobial Resistance Profiles of Staphylococcus aureus from Maine Dairy Farms

Staphylococcus aureus is a bacterial pathogen which causes mastitis in dairy livestock. This study described retrospective trends of S. aureus detection in milk samples submitted between July 2017 and June 2022 to the University of Maine Cooperative Extension Veterinary Diagnostic Laboratory and assessed genomes of 29 S. aureus isolates. S. aureus was detected in 7.8% of milk samples from the observed period. The 29 genomes belonged to 8 strain types and 5 clonal complexes and contained antimicrobial resistance and exotoxin genes which mostly clustered with clonal complexes and species of origin. Future research should assess S. aureus prevalence in Maine.