Novel Nec-1 Mimics as Antioxidant Peptides and Modifications to the FOX Assay
Conference Year
2024
Abstract
Ergothioneine is a natural amino acid with powerful antioxidant properties. While ergothioneine has a trimethylated alpha-nitrogen which prevents its incorporation into a peptide, its synthetic analogue 2-thiohistidine shares ergothioneine’s antioxidant properties and can be incorporated into a peptide. This project involves incorporating 2-thiohistidine into experimental dipeptide mimics of Necrostatin-1, a small molecule which protects against oxidative stress associated with cell death. To measure the ability of the Necrostatin-1 mimics (W-2TH, W-H and W-Q) to protect against oxidative damage, this project also involves designing an assay to measure lipid peroxidation with high efficiency and specificity, based on the ferrous xylenol-orange assay.
Primary Faculty Mentor Name
Robert Hondal
Status
Undergraduate
Student College
College of Agriculture and Life Sciences
Program/Major
Biochemistry
Primary Research Category
Life Sciences
Novel Nec-1 Mimics as Antioxidant Peptides and Modifications to the FOX Assay
Ergothioneine is a natural amino acid with powerful antioxidant properties. While ergothioneine has a trimethylated alpha-nitrogen which prevents its incorporation into a peptide, its synthetic analogue 2-thiohistidine shares ergothioneine’s antioxidant properties and can be incorporated into a peptide. This project involves incorporating 2-thiohistidine into experimental dipeptide mimics of Necrostatin-1, a small molecule which protects against oxidative stress associated with cell death. To measure the ability of the Necrostatin-1 mimics (W-2TH, W-H and W-Q) to protect against oxidative damage, this project also involves designing an assay to measure lipid peroxidation with high efficiency and specificity, based on the ferrous xylenol-orange assay.