Photoablation-based Treatment for Bacterial Keratitis Using Immunogold Bioconjugate Nanoparticles
Conference Year
2024
Abstract
Bacterial keratitis, a vision-threatening corneal infection, can rapidly progress without the proper treatment, currently consisting of high frequency antibiotic eyedrop application. We aim to develop a more efficient pulsed plasmonic laser photoablation (PPLP) based therapy utilizing the unique optical properties of gold nanoparticles and the active targeting of Pseudomonas aeruginosa, a common cause of keratitis. The resulting immunogold bioconjugate nanoparticles were characterized based on size and zeta potential analysis. The efficiency of attachment to bacterial cells was investigated by varying the nanoparticle-to-bacteria ratio and incubation temperature. Nanoparticle attachment to the surface of bacteria was quantified using spectroscopy and microscopy techniques.
Primary Faculty Mentor Name
Amber Doiron
Status
Graduate
Student College
College of Engineering and Mathematical Sciences
Program/Major
Biomedical Engineering
Primary Research Category
Engineering and Math Science
Photoablation-based Treatment for Bacterial Keratitis Using Immunogold Bioconjugate Nanoparticles
Bacterial keratitis, a vision-threatening corneal infection, can rapidly progress without the proper treatment, currently consisting of high frequency antibiotic eyedrop application. We aim to develop a more efficient pulsed plasmonic laser photoablation (PPLP) based therapy utilizing the unique optical properties of gold nanoparticles and the active targeting of Pseudomonas aeruginosa, a common cause of keratitis. The resulting immunogold bioconjugate nanoparticles were characterized based on size and zeta potential analysis. The efficiency of attachment to bacterial cells was investigated by varying the nanoparticle-to-bacteria ratio and incubation temperature. Nanoparticle attachment to the surface of bacteria was quantified using spectroscopy and microscopy techniques.