Epigenetic mechanisms of IKAROS tumor suppressor in acute lymphoblastic leukemia

Presenter's Name(s)

Alyssa R. RichmanFollow

Conference Year

2022

Abstract

Hematopoietic malignancies are defined by genetic and epigenetic aberrations. The hematopoietic transcription factor IKAROS, encoded by IKZF1, coordinates chromatin structure to promote normal hematopoietic gene networks and is commonly mutated in precursor B cell acute lymphoblastic leukemia (preB-ALL). In patient derived cell models of preB-ALL, rescue of IKAROS function leads to cell cycle arrest; however, the mechanism behind IKAROS tumor suppression is incompletely understood. Here we investigate epigenetic deregulation in preB-ALL patient cell models to elucidate the mechanism of IKAROS tumor suppression. We identify alterations in global DNA methylation and chromatin accessibility patterns which may promote leukemogenic gene networks.

Primary Faculty Mentor Name

Seth Frietze

Graduate Student Mentors

Princess Rodriguez, PhD, Hana Paculova, PhD

Faculty/Staff Collaborators

Joseph Boyd, MS, Hilde Schjerven, PhD

Status

Graduate

Student College

College of Nursing and Health Sciences

Program/Major

Cellular, Molecular and Biomedical Sciences

Primary Research Category

Biological Sciences

Abstract only.

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Epigenetic mechanisms of IKAROS tumor suppressor in acute lymphoblastic leukemia

Hematopoietic malignancies are defined by genetic and epigenetic aberrations. The hematopoietic transcription factor IKAROS, encoded by IKZF1, coordinates chromatin structure to promote normal hematopoietic gene networks and is commonly mutated in precursor B cell acute lymphoblastic leukemia (preB-ALL). In patient derived cell models of preB-ALL, rescue of IKAROS function leads to cell cycle arrest; however, the mechanism behind IKAROS tumor suppression is incompletely understood. Here we investigate epigenetic deregulation in preB-ALL patient cell models to elucidate the mechanism of IKAROS tumor suppression. We identify alterations in global DNA methylation and chromatin accessibility patterns which may promote leukemogenic gene networks.