Revealing two distinct molecular binding modes in polyethylenimine-DNA polyplexes using infrared spectroscopy
Abstract
Non-viral polymeric delivery vectors have attracted significant attention due to their reduced immunogenic effects. However, their efficiency and efficacy are poorly understood. A crucial factor is the formation of the polymer-DNA complex, which should be strong enough to avoid cellular degradation, but weak enough to allow efficient DNA release. To address this, we utilized FTIR spectroscopy to develop a molecular-level study by probing the structures of two polyethyleneimine systems. Our findings provide essential insights into the behavior of these interactions at a molecular level, paving the way for designing more effective polymer systems.
Primary Faculty Mentor Name
David Punihaole
Status
Undergraduate
Student College
College of Arts and Sciences
Program/Major
Biochemistry
Primary Research Category
Physical Science
Revealing two distinct molecular binding modes in polyethylenimine-DNA polyplexes using infrared spectroscopy
Non-viral polymeric delivery vectors have attracted significant attention due to their reduced immunogenic effects. However, their efficiency and efficacy are poorly understood. A crucial factor is the formation of the polymer-DNA complex, which should be strong enough to avoid cellular degradation, but weak enough to allow efficient DNA release. To address this, we utilized FTIR spectroscopy to develop a molecular-level study by probing the structures of two polyethyleneimine systems. Our findings provide essential insights into the behavior of these interactions at a molecular level, paving the way for designing more effective polymer systems.