Identification of Molecular Mechanisms of Sema6A Reverse Signaling in Eye Development
Conference Year
January 2022
Abstract
Sema6a is a signaling molecule that plays an important role in the development of tissues including the brain and eye. Sema6a functions as a ligand in a forward signaling cascade, but it can also serve as a receptor in reverse signaling. The mechanism of Sema6a reverse signaling is poorly understood but recent research has found that the tyrosine kinase Abl interacts with the Sema6a intracellular domain and may be essential for downstream phosphorylation. In this project, I identified tyrosine phosphorylation sites and binding partners on Sema6a’s intracellular domain in the presence of Abl using cell culture and mass spectrometry analysis
Primary Faculty Mentor Name
Bryan Ballif
Graduate Student Mentors
Caroline Dumas
Status
Undergraduate
Student College
College of Agriculture and Life Sciences
Program/Major
Neuroscience
Primary Research Category
Biological Sciences
Identification of Molecular Mechanisms of Sema6A Reverse Signaling in Eye Development
Sema6a is a signaling molecule that plays an important role in the development of tissues including the brain and eye. Sema6a functions as a ligand in a forward signaling cascade, but it can also serve as a receptor in reverse signaling. The mechanism of Sema6a reverse signaling is poorly understood but recent research has found that the tyrosine kinase Abl interacts with the Sema6a intracellular domain and may be essential for downstream phosphorylation. In this project, I identified tyrosine phosphorylation sites and binding partners on Sema6a’s intracellular domain in the presence of Abl using cell culture and mass spectrometry analysis