Identification of Molecular Mechanisms of Sema6A Reverse Signaling in Eye Development

Abstract

Sema6a is a signaling molecule that plays an important role in the development of tissues including the brain and eye. Sema6a functions as a ligand in a forward signaling cascade, but it can also serve as a receptor in reverse signaling. The mechanism of Sema6a reverse signaling is poorly understood but recent research has found that the tyrosine kinase Abl interacts with the Sema6a intracellular domain and may be essential for downstream phosphorylation. In this project, I identified tyrosine phosphorylation sites and binding partners on Sema6a’s intracellular domain in the presence of Abl using cell culture and mass spectrometry analysis

Primary Faculty Mentor Name

Bryan Ballif

Graduate Student Mentors

Caroline Dumas

Status

Undergraduate

Student College

College of Agriculture and Life Sciences

Program/Major

Neuroscience

Primary Research Category

Biological Sciences

Abstract only.

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Identification of Molecular Mechanisms of Sema6A Reverse Signaling in Eye Development

Sema6a is a signaling molecule that plays an important role in the development of tissues including the brain and eye. Sema6a functions as a ligand in a forward signaling cascade, but it can also serve as a receptor in reverse signaling. The mechanism of Sema6a reverse signaling is poorly understood but recent research has found that the tyrosine kinase Abl interacts with the Sema6a intracellular domain and may be essential for downstream phosphorylation. In this project, I identified tyrosine phosphorylation sites and binding partners on Sema6a’s intracellular domain in the presence of Abl using cell culture and mass spectrometry analysis