Investigating HIV-1 Vpu-Mediated Downregulation of EWI-2

Abstract

EWI-2 and CD81 are fusion inhibitory proteins, preventing excessive HIV-1-induced cell-cell fusion by localizing at the virological synapse (VS); A cellular junction allowing for efficient cell-to-cell virus transmission. CD81 and EWI-2 are downregulated, yet needed at the VS, revealing a delicate balance that needs to be maintained to maximize virus spread. Fluorescent microscopy co-localization experiments showed a high degree of co-localization between EWI-2 and Vpu. Flow cytometry analysis of tetraspanin knockdown HeLa cells transfected with Vpu and stably expressing EWI-2 will reveal any differences in surface EWI-2 levels when CD81 levels are impacted.

Primary Faculty Mentor Name

Markus Thali

Status

Undergraduate

Student College

College of Agriculture and Life Sciences

Program/Major

Microbiology and Molecular Genetics

Primary Research Category

Biological Sciences

Secondary Research Category

Health Sciences

Abstract only.

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Investigating HIV-1 Vpu-Mediated Downregulation of EWI-2

EWI-2 and CD81 are fusion inhibitory proteins, preventing excessive HIV-1-induced cell-cell fusion by localizing at the virological synapse (VS); A cellular junction allowing for efficient cell-to-cell virus transmission. CD81 and EWI-2 are downregulated, yet needed at the VS, revealing a delicate balance that needs to be maintained to maximize virus spread. Fluorescent microscopy co-localization experiments showed a high degree of co-localization between EWI-2 and Vpu. Flow cytometry analysis of tetraspanin knockdown HeLa cells transfected with Vpu and stably expressing EWI-2 will reveal any differences in surface EWI-2 levels when CD81 levels are impacted.