Determining the Relationship of Peptide’s Ramachandran φ Angle and the Raman Amide III2 Frequency
Conference Year
2024
Abstract
Raman spectroscopy, an inelastic light scattering technique, is a tool that can probe molecular vibrations of proteins. Raman spectroscopy can quantify a protein’s averaged Ramachandran -angles due to its relationship with the Amide III vibrational mode. However, a relationship between the Ramachandran -angle and a vibrational mode has not been determined. We developed a method to crystallize dipeptides with varying Ramachandran -angles to correlate the -angles to the Amide III vibrational mode. This relationship will be applied to enhance the structural characterization of amyloid fibril protein aggregates, which are implicated in Alzheimer’s disease.
Primary Faculty Mentor Name
David Punihaole
Graduate Student Mentors
Madeline Harper
Status
Undergraduate
Student College
College of Arts and Sciences
Program/Major
Biochemistry
Primary Research Category
Physical Science
Determining the Relationship of Peptide’s Ramachandran φ Angle and the Raman Amide III2 Frequency
Raman spectroscopy, an inelastic light scattering technique, is a tool that can probe molecular vibrations of proteins. Raman spectroscopy can quantify a protein’s averaged Ramachandran -angles due to its relationship with the Amide III vibrational mode. However, a relationship between the Ramachandran -angle and a vibrational mode has not been determined. We developed a method to crystallize dipeptides with varying Ramachandran -angles to correlate the -angles to the Amide III vibrational mode. This relationship will be applied to enhance the structural characterization of amyloid fibril protein aggregates, which are implicated in Alzheimer’s disease.