CRISPR technology aids in the investigation of Rab6 involvement with Endoplasmic Reticulum.
Conference Year
2024
Abstract
The largest organelle in our cells, the endoplasmic reticulum (ER), consists of interconnected membrane tubules. The ER plays an essential role in many cellular functions including protein synthesis and secretion, and ER defects are linked to diseases such as cancer and neurodegeneration. ER tubules are highly motile, and a novel mode of ER movement called “hitchhiking” relies on interactions between ER tubules and Rab-vesicles. Through systematic screening, we found that ER hitchhikes on vesicles marked by Rab6. Bioengineering and CRISPR genome editing were used to characterize the necessity and sufficiency of Rab6 in ER hitchhiking and tubule movement.
Primary Faculty Mentor Name
John Salogiannis
Graduate Student Mentors
Ally Morrisey, Sarah Abeling
Status
Undergraduate
Student College
College of Arts and Sciences
Program/Major
Biology
Primary Research Category
Life Sciences
CRISPR technology aids in the investigation of Rab6 involvement with Endoplasmic Reticulum.
The largest organelle in our cells, the endoplasmic reticulum (ER), consists of interconnected membrane tubules. The ER plays an essential role in many cellular functions including protein synthesis and secretion, and ER defects are linked to diseases such as cancer and neurodegeneration. ER tubules are highly motile, and a novel mode of ER movement called “hitchhiking” relies on interactions between ER tubules and Rab-vesicles. Through systematic screening, we found that ER hitchhikes on vesicles marked by Rab6. Bioengineering and CRISPR genome editing were used to characterize the necessity and sufficiency of Rab6 in ER hitchhiking and tubule movement.