Impact of STK11 loss-of-function mutations on the metastatic potential of KRAS-mutated lung-adenocarcinoma
Conference Year
2024
Abstract
This study aimed to determine the impact of STK11 loss-of-function mutations on the metastatic potential in KRAS-mutated lung adenocarcinomas. The metastatic potential of STK11 loss-of-function lines were assessed utilizing migration as well as invasion assays. H2009 and ∆STK11, previously generated by CRISPR/Cas9 were utilized. ∆STK11 cells displayed a significant increase in migration compared to the parental control (n=6; p<0.0001). The invasive properties of ∆STK11 cells were assessed utilizing spheroids embedded in Matrigel matrix. The area of invasion in 24 hours was significantly larger in ∆STK11 cells compared to the parental control (n=19-21; p<0.0001).
Primary Faculty Mentor Name
Melissa Scheiber
Status
Undergraduate
Student College
College of Nursing and Health Sciences
Program/Major
Medical Laboratory Science
Primary Research Category
Clinical
Impact of STK11 loss-of-function mutations on the metastatic potential of KRAS-mutated lung-adenocarcinoma
This study aimed to determine the impact of STK11 loss-of-function mutations on the metastatic potential in KRAS-mutated lung adenocarcinomas. The metastatic potential of STK11 loss-of-function lines were assessed utilizing migration as well as invasion assays. H2009 and ∆STK11, previously generated by CRISPR/Cas9 were utilized. ∆STK11 cells displayed a significant increase in migration compared to the parental control (n=6; p<0.0001). The invasive properties of ∆STK11 cells were assessed utilizing spheroids embedded in Matrigel matrix. The area of invasion in 24 hours was significantly larger in ∆STK11 cells compared to the parental control (n=19-21; p<0.0001).