Optimization of an Embryonic Zebrafish Xenograft Model of Metastasis
Conference Year
2024
Abstract
The zebrafish xenograft model is valuable for studying cancer metastasis but lacks temperature standardization. Thus, our study aimed to establish an optimal temperature and migratory controls. Wild-type zebrafish embryos incubated at 34°C, 35°C and 28.5°C showed significantly lower mortality at 28.5°C after 6 days. While 34°C displayed significant mortality, embryo morphology was not impacted as seen at 35°C, consistent with previous findings. Fluorescently labeled human cancer cells are currently being injected into transgenic zebrafish embryos and imaged at 1- and 5-days post-injection to quantify extravasation. This will aid in exploring the impact of mutations like STK11 on lung adenocarcinoma metastasis.
Primary Faculty Mentor Name
Melissa Scheiber
Status
Undergraduate
Student College
College of Nursing and Health Sciences
Program/Major
Medical Laboratory Science
Primary Research Category
Life Sciences
Optimization of an Embryonic Zebrafish Xenograft Model of Metastasis
The zebrafish xenograft model is valuable for studying cancer metastasis but lacks temperature standardization. Thus, our study aimed to establish an optimal temperature and migratory controls. Wild-type zebrafish embryos incubated at 34°C, 35°C and 28.5°C showed significantly lower mortality at 28.5°C after 6 days. While 34°C displayed significant mortality, embryo morphology was not impacted as seen at 35°C, consistent with previous findings. Fluorescently labeled human cancer cells are currently being injected into transgenic zebrafish embryos and imaged at 1- and 5-days post-injection to quantify extravasation. This will aid in exploring the impact of mutations like STK11 on lung adenocarcinoma metastasis.